The KanR gene is flanked by 19 bp inverted repeats mosaic ends, MEswhich serve as transposase recognition sequences for excision and insertion of the transposon [ 1 ]. Then, using a sterile loop for each tube, l were pipetted into the appropriate plates.
From your results, can you tell if these bacteria are ampicillin resistant by looking at them on the LB plate? The organism can adapt to conditions and wont have to waste and spend energy producing unnecessary proteins.
There was no fluorescence visible when the UV light was shined on the plasmid in the tube because it is not the plasmid that reacts to the light but the protein produced when the gene in the plasmid is translated.
Is there a difference? We will be using the bacterium Escherichia coli E.
LB had a white lawn of bacteria. Transformation efficiency is the quantitative value that describes how effective the transfer of plasmids into bacteria.
Then the gene is transcribed. Compare the growth on plates 2 and 4 and then explain your answer. The main objective of the procedures to be explored will be to familiarize or increase the laboratory proficiency of biology students and students from other majors with some of the basic techniques that underlie microbiological and molecular biological research.
A What two factors must be present in the bacterias environment for you to see the green color?