Bacterial genetics worksheet

Viral and bacterial genetics worksheet answers

Illustration of the circular DNA created in step 2. Spray workspace with bleach solution and wipe with paper towels. In the laboratory, DNA fragments of interest can be engineered into phagemids, which are plasmids that have phage sequences that allow them to be packaged into bacteriophages. Why do only transformants grow on this plate? Figure 1. Johns Hopkins University Press, Baltimore. Viral genes can be deleted and replaced with the gene to be delivered to the patient; [3] the virus then infects the host cell and delivers the foreign DNA into the genome of the targeted cell.

Were all of the bacteria that we started with transformed? Both tubes should now be on ice.

Bacterial genetics worksheet

Micrometers are one millionth of a meter. DO NOT add the plasmid to your "-" tube. Answer: most are only a few micrometers. Let them stay on ice for at least one minute. What is cDNA? Clean up: Place used loops etc in the bacterial waste container.

Close lids, and gently tap tubes to mix. This process is called transformation.

microbial genetics ppt

The common method used for genetically modifying bacteria is to use recombinant plasmids. On plate 3 the untransformed bacteria are unable to grow in the presence of the antibiotic since bacterial cell walls are unable to form and the bacterial cells die.

Transformation transduction and conjugation in bacteria ppt

Compare the growth on plates 2 and 4 and then explain your answer. This sequence and the cut pattern are shown in Figure 3. These fragments can be used to determine the sequence of the DNA and the function of any genes present. Other changes to bacteria can reconfigure the cellular respiration product to create desirable byproducts such as diesel or plastic molecules instead of the usual byproduct, such as carbon dioxide. Which one is it? Researchers have been able to manipulate these naturally occurring plasmids to remove their tumor-causing genes and insert desirable DNA fragments. In the laboratory, DNA fragments of interest can be engineered into phagemids, which are plasmids that have phage sequences that allow them to be packaged into bacteriophages. Using plasmids in the lab Plasmids can be used as vectors to carry foreign DNA into a cell. The resulting transformed cells are allowed to recover and can be used to generate recombinant plants. Let's look at how we can modify these bacteria and why we would want to modify them. Transformed bacteria can then be grown in large amounts.

The process to build a recombinant plasmid to modify bacteria. Explain what you think might have happened.

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Bacterial transformation